Zeiss LSM 880 NLO Fast Airyscan Confocal

A multifunctional system suited for studying diseases in their host environment.

Overview

The Zeiss LSM 880 NLO with fast Airyscan Confocal microscope is a well-equipped multi-photon system suitable for in vivo and in vitro studies of disease.

The system is equipped with a MaiTai DeepSee multi-photon laser tuneable from 690 nm – 1040 nm. This provides an excellent system well suited to intravital microscopy.

The microscope also features an Airyscan detection unit for an improved resolution of 1.7x that of diffraction limited confocal microscopy.

Examples of work
  • Photo of the Zeiss LSM 880 microscope
    A Zeiss LSM 880 microscope at the Institute
  • Microscopy image of intestinal villi
    Intestinal villi imaged on the Zeiss LSM 880 microscope. Credit: Caleb Dawson
  • Microscopy image of red blood cell
    A group of red blood cells (magenta) infected by a malaria-causing parasite. Parasite DNA is shown in blue, some parasite proteins are shown in green. Credit: Michał Pasternak

Hardware

Objective size

20

25

40

63

NA

1

0.8

1.3

1.4

Immersion media

Water (dipping)

Multi
Water/Glycerine/Oil

Oil

Oil

XY-resolution (μm) (@ 488 nm)

0.297

0.372/0.372/0.372

0.229

0.212

Z-resolution (µm) (@ 488 nm)

1.3

2.031/2.246/2.314

0.876

0.755

Working distance (mm)

1.7

0.570

0.210

0.190

Light sources

  • Diode laser – 405 nm
  • Argon laser – 458 nm, 488 nm, 594 nm
  • DPSS – 561 nm
  • HeNe – 633 nm
  • MaiTai DeepSee – 690 nm – 1040 nm

Detection source

  • 2 x Big (GaAsP) NDD detectors
  • 2 x NDD detectors
  • 1 x 32 channel GaAsP array
  • 1 x Airyscan Detector

Technological specifications (capabilities)

Available modalities

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions
  • FRAP
  • Spectral unmixing
  • Online fingerprinting

Additional hardware

  • Airyscan detection
  • Fast Airyscan module

Unique features

The Zeiss LSM 880 NLO plus fast Airyscan Confocal microscope is a multi-functional two-photon microscope with numerous detection options including the Zeiss Airyscan and fast Airyscan modules for high resolution and high speed imaging respectively.

Limitations

  • Not suited for live cellular samples
  • Photobleaching