A high speed, high resolution microscope for investigating dynamic processes in living cells.
Overview
The Zeiss LSM 880 with fast Airyscan confocal microscope is the essential confocal platform for live cell imaging. The unique combination of the Airyscan detector module with the Definite Focus.2 and Z-Piezo drive offers unrivalled flexibility in fast, high resolution live cell imaging.
The system is compatible with most sample holders and contains all necessary accessories for environmental control (for example, CO2, humidity, and temperature control).
Examples of work
Hardware
Magnification | 10x | 20x | 40x | 63x | 63x |
NA | 0.45 | 0.8 | 1.3 | 1.4 | 1.2 |
Immersion media | Air | Air | Oil | Oil | Water |
XY-resolution (µm) (@ 488 nm) | 0.661 | 0.372 | 0.229 | 0.212 | 0.248 |
Z-resolution (µm) (@ 488 nm) | 4.819 | 1.524 | 0.876 | 0.755 | 0.902 |
Working distance (mm) | 2.000 | 0.550 | 0.21 | 0.140 | 0.28 |
Light sources
- Diode laser – 405 nm
- Argon laser – 458 nm, 488 nm and 514 nm
- Diode pumped solid state laser – 561 nm
- HeNe Laser – 594 nm
- HeNe laser – 633 nm
- HAL 100 Illuminator
- HXP-120 120 V
Detection sources
- 32 channel GaAsP array detection
- 2 x MA-PMT
- Airyscan detector
- Transmitted-PMT
Technological specifications (capabilities)
Available modalities
- Z-stack
- Time-series
- Tile scan
- Multi positions
- Spectral linear unmixing
- Online fingerprinting
- Single point FCS
- FRAP
- Airyscan detection
- Fast Airyscan
Additional hardware
- Z-Piezo drive
- Definite Focus.2
Unique features
- The Zeiss LSM 880 combines the fast Airyscan modality with the Z-Piezo drive system to offer fast live cell three-dimensional imaging.
- The Definite Focus.2 module provides one of the most robust systems for live time-lapse imaging.
Limitations
- Photobleaching