Zeiss Axio Observer (Airlock)

The Zeiss Axio Observer is a flexible inverted microscope that allows scientists to view a range of samples under a variety of conditions.

Overview

The Zeiss Axio Observer (Airlock) is an inverted modular microscope that allows for flexible and automated experimental design. With this microscope, scientists can create optimal conditions for a range of samples, from living cells to fixed tissues.

The Axio Observer system is suitable for both labelled and label-free samples. The system contains a full colour camera for imaging of Immunohistochemistry stained samples.  

It is compatible with a variety of sample chambers, including: 

  • two to eight well chamber slides
  • petri dishes
  • multi-well plates
Examples of work
  • Zeiss Axio Observer (Airlock) microscope
  • Microsopy images of toxoplasma parasites
    As the Toxoplasma parasite grows inside a cell, it forms patterns that look like flowers, called rosettes. Simona Seizova is testing new techniques to disable Toxoplasma parasites, as a way to find new treatments for people with toxoplasmosis. These images help to test how effective the techniques are at killing the parasite. Credit: Simona Seizova
  • Snapshot of fat cells
    Cultured adipocytes (fat cells), derived from progenitor cells. Credit: Maria Bergamasco

Hardware

Objective magnification 5x 10x 20x 40x 63x 100x
Immersion media Air Air Air Water Oil Oil
XY-resolution (µm) 2.22 0.74 0.42 0.28 0.24 0.24
Working distance (mm) 12 2.0 0.55 0.28 0.19 0.17
NA 0.15 0.45 0.8 1.2 1.4 1.4

Light sources

  • LED Illumination: 470 nm, 555 nm and 625 nm
  • Illuminator HXP 120 V (DAPI, GFP, CY3, Texas Red and Cy5 nominal filter sets
  • Bright field illumination (Phase contrast and DIC)

Detection source

  • PCO.Edge sCMOS 16-bit camera 
  • Zeiss Axio cam colour

Technological specifications (capabilities)

Available modalities

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions

Additional hardware

  • Definite focus
  • Open application development for automated workflow

Unique features

The microscope features a full colour camera for imaging of immunostained slides. 

Limitations

  • Limited 3D resolution
  • Poor signal-to-noise ratio in brightly labelled samples
  • Photobleaching

ACKNOWLEDGEMENT

This microscope was purchased with the generous support of the Harry Secomb Trust.