DeltaVision Elite

The Applied Precision (GE) DeltaVision Elite is a widefield microscope designed to take advantage of in-built widefield deconvolution algorithms. 

Overview

The DeltaVision Elite is an inverted fluorescent microscope that allows efficient collection and processing of widefield deconvolution data. Widefield deconvolution is a mathematical process whereby signal to noise and resolution of widefield data is improved by taking into account the point spread function (PSF) of the imaging system and correcting for any aberrations of the optics. 

The system can accommodate slides (or chamber slides) or 35mm dishes. This system is suited to fixed imaging as there is currently no environmental control installed. 

This microscope will allow three-dimensional measurements at higher resolution than traditional widefield microscopes. 

Examples of work
  • Deltavision Elite microscope
    The Deltavision Elite microscope at the Institute
  • Microscopy image of lung cancer cell
    Lung squamous cell cancer cut into thin sections to see individual cells inside the tumour. Fluorescent in situ hybridization (FISH) was conducted using a probe for MCL-1 labelled in Spectrum Orange and using a control marker for Chromosome 1, labelled in Spectrum Green. DAPI was used to stain nuclei. Imaging was conducted on the DeltaVision Elite microscope. Credit: Casey Ah-Cann
  • Microscopy image of lung cancer cell
    Lung squamous cell cancer cut into thin sections to see individual cells inside the tumour. Fluorescent in situ hybridization (FISH) was conducted using a probe for MCL-1 labelled in Spectrum Orange and using a control marker for Chromosome 1, labelled in Spectrum Green. DAPI was used to stain nuclei. Imaging was conducted on the DeltaVision Elite microscope. Credit: Casey Ah-Cann
  • Microscopic image of gout-causing crystals
    Monosodium urate crystals, the cause of gout, are shown caught in a web of DNA and protein released by a type of human immune cell, the neutrophil. In this image cells are stained with antibodies against neutrophil elastase (green) and myeloperoxidase (red). The cells are also stained with DAPI (blue) to image the DNA backbone of these structures and the fluorescent images are merged with a differential interference contrast image. Credit: Simon Chatfield

Hardware

Objective magnification 2x 10x 20x 40x 60x 100x
Immersion media Air Air Air Water Oil Oil
XY - resolution (µm) 3.7 0.75 .41 0.33 0.21 0.21
Working distance (mm) 6.2 3.1 0.6 0.2 0.15 0.13
NA 0.08 0.4 0.75 0.9 1.42 1.4

Light sources

  • LED Illumination with matched filter sets:
  • DAPI/FITC/TRITC/Cy5
  • DAPI/FITC/A594/Cy5
  • Bright field illumination (DIC)

Detection source

  • Photometrics Coolsnap HQ2 – 12bit CCD

Technological specifications (capabilities)

Available modalities

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions

Additional capabilities

  • Inbuilt deconvolution 
  • Batch Processing
  • Sample mapping between DV and OMX

Unique features

  • The softWoRx software runs both the acquisition and processing of data on the system. 
  • Deconvolution can be batch processed using already measured point spread functions for optimal computational accuracy. 
  • The stage can be mapped to the OMX SR super-resolution system. This allows sample location to be performed on the DeltaVision Elite before imaging using structured illumination on the OMX SR system. 

Limitations

  • Currently no environmental controls 
  • 12-bit camera has limited dynamic range
  • Limited z-sectioning capabilities (although better than standard widefield)

Acknowledgement

This microscope was purchased with the support of the Joe White Bequest.