The DeltaVision Elite is an inverted fluorescent microscope that allows efficient collection and processing of widefield deconvolution data. Widefield deconvolution is a mathematical process whereby signal to noise and resolution of widefield data is improved by taking into account the point spread function (PSF) of the imaging system and correcting for any aberrations of the optics.
The system can accommodate slides (or chamber slides) or 35mm dishes. This system is suited to fixed imaging as there is currently no environmental control installed.
This microscope will allow three-dimensional measurements at higher resolution than traditional widefield microscopes.
|XY - resolution (µm)||3.7||0.75||.41||0.33||0.21||0.21|
|Working distance (mm)||6.2||3.1||0.6||0.2||0.15||0.13|
- LED Illumination with matched filter sets:
- Bright field illumination (DIC)
- Photometrics Coolsnap HQ2 – 12bit CCD
Technological specifications (capabilities)
- Tile scan
- Multi positions
- Inbuilt deconvolution
- Batch Processing
- Sample mapping between DV and OMX
- The softWoRx software runs both the acquisition and processing of data on the system.
- Deconvolution can be batch processed using already measured point spread functions for optimal computational accuracy.
- The stage can be mapped to the OMX SR super-resolution system. This allows sample location to be performed on the DeltaVision Elite before imaging using structured illumination on the OMX SR system.
- Currently no environmental controls
- 12-bit camera has limited dynamic range
- Limited z-sectioning capabilities (although better than standard widefield)
This microscope was purchased with the support of the Joe White Bequest.