A next generation confocal for fast and gentle 4D imaging.
Overview
The Zeiss LSM 980 with Airyscan 2 confocal microscope at the Walter and Eliza Hall Institute is one of the first available in the world. The new Airyscan detector module with the Definite Focus.2 and Z-Piezo drive offers unrivalled flexibility in fast, high resolution live cell imaging in a familiar confocal setup.
The system is compatible with most sample holders and contains all necessary accessories for environmental control, for example, CO2, humidity and temperature control.
Examples of work
Zeiss LSM 980 with Airyscan 2 microscope
Objectives
| Magnification | 10x | 20x | 40x | 63x | 63x |
| NA | 0.45 | 0.8 | 1.3 | 1.4 | 1.2 |
| Immersion media | Air | Air | Oil | Oil | Water |
| XY-resolution (µm) (@ 488 nm) | 0.661 | 0.372 | 0.229 | 0.212 | 0.248 |
| Z-resolution (µm) (@ 488 nm) | 4.819 | 1.524 | 0.876 | 0.755 | 0.902 |
| Working distance (mm) | 2.000 | 0.550 | 0.21 | 0.140 | 0.28 |
Light sources
- Diode laser – 405 nm
- Argon laser – 445 nm, 488 nm and 514 nm
- Diode pumped solid state laser – 561 nm
- HeNe Laser – 594 nm
- HeNe laser – 639 nm
- HAL 100 Illuminator
- HXP-120 120 V
Detection source
- 32 channel GaAsP array detection
- 2 x MA-PMT
- Airyscan 2 detector
- Transmitted-PMT
Technological specifications (capabilities)
Available modalities
- Z-stack
- Time-series
- Tile scan
- Multi positions
- Spectral linear unmixing
- Online fingerprinting
- FRAP
- Airyscan 2 detection
- Fast Airyscan
Unique features
The brand new Airyscan 2 detector enables the fastest point-scanning confocal system on the market. Operating at up to 10 fps with the resolution of standard confocal or better with high Signal-to-Noise.
Limitations
- Photobleaching
