Overview
The Zeiss LSM 880 NLO with fast Airyscan Confocal microscope is a well-equipped multi-photon system suitable for in vivo and in vitro studies of disease.
The system is equipped with a MaiTai DeepSee multi-photon laser tuneable from 690 nm – 1040 nm. This provides an excellent system well suited to intravital microscopy.
The microscope also features an Airyscan detection unit for an improved resolution of 1.7x that of diffraction limited confocal microscopy.
-
A Zeiss LSM 880 microscope at the Institute -
To image this whole lymph node in 3D, the organ was rendered optically transparent to permit a better penetration of the light for fluorescence microscopy imaging. Credit: Fanny Lafouresse and Verena Wimmer -
High resolution 3D imaging showing tumour cells induce the formation of new blood vessels to facilitate their growth within bone marrow. Credit: Raymond Yip -
This image captures various immune cells, identified by membrane staining, interacting together within a lymph node. Credit: Fanny Lafouresse and Verena Wimmer -
Cross section of a developing five-day old zebrafish intestine. Single mitochondria can be seen as small green donuts throughout the image. DNA within the nucleus (blue) and actin cytoskeleton (red) enable us to identify single cells. Credit: Stephen Mieruszynski -
3D imaging enables researchers to visualise bone metastasis progression at single-cell resolution. Credit: Raymond Yip -
A group of red blood cells (magenta) infected by a malaria-causing parasite. Parasite DNA is shown in blue, some parasite proteins are shown in green. Credit: Michał Pasternak -
Intestinal villi imaged on the Zeiss LSM 880 microscope. Credit: Caleb Dawson
-
A Zeiss LSM 880 microscope at the Institute
-
To image this whole lymph node in 3D, the organ was rendered optically transparent to permit a better penetration of the light for fluorescence microscopy imaging. Credit: Fanny Lafouresse and Verena Wimmer
-
High resolution 3D imaging showing tumour cells induce the formation of new blood vessels to facilitate their growth within bone marrow. Credit: Raymond Yip
-
This image captures various immune cells, identified by membrane staining, interacting together within a lymph node. Credit: Fanny Lafouresse and Verena Wimmer
-
Cross section of a developing five-day old zebrafish intestine. Single mitochondria can be seen as small green donuts throughout the image. DNA within the nucleus (blue) and actin cytoskeleton (red) enable us to identify single cells. Credit: Stephen Mieruszynski
-
3D imaging enables researchers to visualise bone metastasis progression at single-cell resolution. Credit: Raymond Yip
-
A group of red blood cells (magenta) infected by a malaria-causing parasite. Parasite DNA is shown in blue, some parasite proteins are shown in green. Credit: Michał Pasternak
-
Intestinal villi imaged on the Zeiss LSM 880 microscope. Credit: Caleb Dawson
Hardware
|
Objective size |
20 |
25 |
40 |
63 |
|
NA |
1 |
0.8 |
1.3 |
1.4 |
|
Immersion media |
Water (dipping) |
Multi |
Oil |
Oil |
|
XY-resolution (μm) (@ 488 nm) |
0.297 |
0.372/0.372/0.372 |
0.229 |
0.212 |
|
Z-resolution (µm) (@ 488 nm) |
1.3 |
2.031/2.246/2.314 |
0.876 |
0.755 |
|
Working distance (mm) |
1.7 |
0.570 |
0.210 |
0.190 |
Light sources
- Diode laser – 405 nm
- Argon laser – 458 nm, 488 nm, 594 nm
- DPSS – 561 nm
- HeNe – 633 nm
- MaiTai DeepSee – 690 nm – 1040 nm
Detection source
- 2 x Big (GaAsP) NDD detectors
- 2 x NDD detectors
- 1 x 32 channel GaAsP array
- 1 x Airyscan Detector
Technological specifications (capabilities)
Available modalities
- Z-stack
- Time-series
- Tile scan
- Multi positions
- FRAP
- Spectral unmixing
- Online fingerprinting
Additional hardware
- Airyscan detection
- Fast Airyscan module
Unique features
The Zeiss LSM 880 NLO plus fast Airyscan Confocal microscope is a multi-functional two-photon microscope with numerous detection options including the Zeiss Airyscan and fast Airyscan modules for high resolution and high speed imaging respectively.
Limitations
- Not suited for live cellular samples
- Photobleaching
