Overview
With the Zeiss Z.1 system scientists can utilise a developing technology to acquire large volumetric data sets of 3D structures.
Unlike traditional widefield and confocal microscopes, the Z.1 utilises a ‘sheet’ of light shone through the sample and the image is then detected perpendicular to the lightsheet.
The Z.1 system is intended for fluorescently labelled samples (both endogenous and antibody labelled). It also has the capability of acquiring a bright-field reference image.
The system comes with a number of specific imaging chambers, however sample preparation and mounting are very flexible. This allows for a wide range of sample types to be imaged.
This microscope provides scientists with the ability to image large ‘cleared’ samples to a much greater depth and at faster speeds than has previously been possible.
Video: 3D rendering of the head of Ascaris, a parasitic worm which resides in the intestines. Credit: Mark Scott, Aaron Jex, Joy Liu
Hardware
Objective magnification | 5x | 20x | 20x |
Immersion media | Air | Water | Clarity |
XY-resolution (µm) | 2.22 | 0.74 | 0.42 |
Working distance (mm) | 18.5 | 2.4 | 5.6 |
NA | 0.16 | 1.0 | 1.0 |
Light sources
- 405, 445, 488, 514, 561 and 638 nm lasers
- Bright field LED illumination
Detection source
- 2 x PCO.Edge sCMOS 16-bit camera
Technological specifications (capabilities)
Available modalities
- Z-stack
- Time-series
- Multi-view (rotation)
Additional options
- Tile scan possible through use of external macro
Limitations
- Resolution limited in Z to lightsheet thickness
- Tile scan and multi-view unable to be set up together