The Nikon N-STORM is a single molecule localisation microscope that is capable of imaging in three-dimensions (3D) with resolutions approximately ten times better than conventional microscopy, typically ~20 nm in xy and ~50 nm in z. The system contains Mic-AO, an adaptive optics module to remove optical aberrations induced by thick samples.
The microscope is also capable of multi-colour, total internal reflection fluorescence (TIRF) and live cell imaging.
The system is compatible with standard slides, 35 mm dishes and 8-well chamber slides. It contains all necessary accessories for environmental control; CO2, humidity, and temperature control.
Staff at the Centre for Dynamic Imaging can provide expert guidance on the preparation of samples and choice of fluorophores.
|Immersion media||Oil||Silicone Oil|
|Working distance (mm)||0.12||0.13|
- 405 nm, 488 nm, 568, 640 nm lasers
- sCMOS cameras (Hamamatsu Orca-Flash4.0 V3)
Technological specifications (capabilities)
- 3D Single Molecule Localization (SML), (also known as PALM, FPALM, STORM, dSTORM, etc)
- 2D SML
- TIRF SML
- Multi-colour SML
- Z-stacks for thick imaging
- Fixed cell imaging
- Live cell imaging
- Tissue imaging
- Perfect Focus for drift-free imaging
- The N-STORM is a stable microscope platform, which enables super-resolution imaging in cells and tissues.
- The Mic-AO adaptive optics system removes optical aberrations to achieve more consistent and uniform resolution throughout thick samples.
- The system enables multi-colour, 3D imaging.
- A 100x/1.45 Silicone Oil objective is also available. Silicone Oil has a refractive index very close to biological specimens.
- Ten to hundreds of thousands of camera frames need to be captured to create one super resolved image.
- Weak staining, excessive bleaching or high levels of background can impair localisation.
- Staff in the Centre for Dynamic Imaging can provide documentation to assist with the preparation of samples.