The Leica SP8 is a high speed, high resolution microscope for investigating dynamic processes in living cells.
Overview
The Leica SP8 Resonant Scanning Confocal microscope is suitable for live cell imaging with subcellular resolution. The system contains a resonant scanning confocal mode allowing for fast imaging of dynamic cellular processes
The system is compatible with most sample holders and contains all necessary accessories for environmental control, for example, CO2, humidity and temperature.
Examples of work
Objectives
Magnification | 10x | 20x | 40x | 40x | 63x | 100x |
NA | 0.4 | 0.75 | 1.3 | 1.1 | 1.4 | 1.4 |
Immersion media | Air | Water/Glycerol | Oil | Water | Oil | Oil |
XY-resolution (µm) (@ 488 nm) | 0.744 | 0.396 | 0.229 | 0.270 | 1.212 | 0.212 |
Z-resolution (µm) (@ 488 nm) | 6.099 | 2.311 | 0.876 | 1.974 | 0.755 | 0.755 |
Working distance (mm) | 2.2 | 0.68 | 0.24 | 0.65 | 0.140 | 0.28 |
Light sources
- Diode laser – 405 nm
- Argon laser – 458 nm, 488 nm and 514 nm
- Diode pumped solid state laser – 561 nm
- HeNe Laser – 594 nm
- HeNe laser – 633 nm
Detection sources
- 3 x PMT
- 2 x HyD detector
Technological specifications (capabilities)
Available modalities
- Z-stack
- Time-series
- Tile scan
- Multi positions
- FRAP
Additional hardware
- FRAP
Unique features
- Resonant scanner for fast imaging
- FRAP module
Limitations
- Photobleaching
- SNR using resonant scanner is poor – requires averaging for low signal
Acknowledgement
This microscope was purchased with the generous support of the Walter and Eliza Hall Trust.